Difference between revisions of "Part:BBa K2442202"

Revision as of 19:54, 1 November 2017

Ptet + RBS + MtlR

This part contains the ptet promoter (R0040) with a strong ribosome binding site (B0032). These components lie upstream of the protein coding sequence for the mtlR protein native to Pseudomonas fluorescens. This part was intended to be used as regulator construct in our system. The mtlR protein is documented to regulate the activity of the native promoter to Pseudomonas , mtle. Currently it is thought that sugars can induce the dimerisation of the mtlR protein and this complex can in turn activate pmtle. This system can be coupled to GFP to create a reporter construct. We tested the regulatory properties of this part by transforming E. coli with the reporter plasmid K2442206. Figure 1 illustrates the result of this and indicates the regulatory property of this part works.

T-Glasgow-finalmtlr.jpg.png

Figure 1 The Regulator (K2442202) + WT reporter construct evoked GFP production with induction achieved by various sugars. In particular when ribose and sorbitol were used as inducers.

Usage and Biology

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

@@ Line 7: / Line 7: @@
 We tested the regulatory properties of this part by transforming <i>E. coli</i> with the reporter plasmid K2442206. <i>Figure 1</i> illustrates the result of this and indicates the regulatory property of this part works.
-https://static.igem.org/mediawiki/parts/a/a5/T-Glasgow-finalmtlr.png
+https://static.igem.org/mediawiki/parts/a/a5/T-Glasgow-finalmtlr.jpg.png
 <i>Figure 1</i> The Regulator (K2442202) + WT reporter construct evoked GFP production with induction achieved by various sugars. In particular when ribose and sorbitol were used as inducers.