Difference between revisions of "Part:BBa K2324004"

Revision as of 19:48, 1 November 2017

FimH+SynMT

The literature has shown that the terminal pili protein FimH (Le Trong et al 2010) can be modified by inserting heterologous sequences at position 225 and 258 (Pallesen et al 1995, Shembri et al 1999). However these two amino acids are in the pilin binding domain which may present difficulties when attempting to introduce large modifications. Harvard iGEM 2015 also introduced modifications at position 1 of the mature FimH protein.

This part codes for a FimH adhesin fused with a metallothionein binding domain from Synechococcus elongatus at amino acid residue 1. It is designed to be placed on the end of a type 1 pilus structure in E. coli with a view to binding a number of heavy metal ions.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal NgoMIV site found at 879
Illegal AgeI site found at 306
1000
COMPATIBLE WITH RFC[1000]

@@ Line 2: / Line 2: @@
 __NOTOC__
 <partinfo>BBa_K2324004 short</partinfo>
-The literature has shown that the terminal pili protein FimH (Le Trong et al 2010) can be modified by inserting heterologous sequences at position 225 and 258 (Pallesen et al 1995, Shembri et al 1999). However these two amino acids are in the pilin binding domain which may present difficulties when attempting to introduce large modifications. Harvard iGEM 2015 also introduced modifications at position 1 of the mature FimH protein.
+<p>
+The literature has shown that the terminal pili protein FimH (Le Trong et al 2010) can be modified by inserting heterologous sequences at position 225 and 258 (Pallesen et al 1995, Shembri et al 1999). However these two amino acids are in the pilin binding domain which may present difficulties when attempting to introduce large modifications. Harvard iGEM 2015 also introduced modifications at position 1 of the mature FimH protein.</p>
 This part codes for a FimH adhesin fused with a metallothionein binding domain from <i>Synechococcus elongatus </i> at amino acid residue 1. It is designed to be placed on the end of a type 1 pilus structure in <i> E. coli </i> with a view to binding a number of heavy metal ions.