Difference between revisions of "Part:BBa K2194003:Design"
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We sourced the nucleotide sequence for <i>cysPUWA</i> from the <i>Escherichia coli</i> MG1655 genome NCBI database (entry NC_000913.3:2539714-2543526). We then sent this sequence to IDT to manufacture as two gBlocks®. | We sourced the nucleotide sequence for <i>cysPUWA</i> from the <i>Escherichia coli</i> MG1655 genome NCBI database (entry NC_000913.3:2539714-2543526). We then sent this sequence to IDT to manufacture as two gBlocks®. | ||
− | [[File:CysPUWA_cds_NCBI.png| | + | [[File:CysPUWA_cds_NCBI.png|600px]] |
===References=== | ===References=== | ||
[update] | [update] |
Revision as of 19:36, 1 November 2017
cysPUWA Sulfate Transport System cds
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 1274
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 2090
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 3
Illegal BsaI.rc site found at 3828
Design Notes
[update]
Source
We sourced the nucleotide sequence for cysPUWA from the Escherichia coli MG1655 genome NCBI database (entry NC_000913.3:2539714-2543526). We then sent this sequence to IDT to manufacture as two gBlocks®.
References
[update]