Difference between revisions of "Part:BBa K2194003:Design"

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<img src="https://static.igem.org/mediawiki/parts/4/41/CysPUWA_cds_NCBI.png" style = "width: 30%">
 
<p class = "caption"> <b>Figure 1:</b> This image is taken from NCBI and shows the <i>cysPUWA</i> genes in their genomic context.</p>
 
<p class = "caption"> <b>Figure 1:</b> This image is taken from NCBI and shows the <i>cysPUWA</i> genes in their genomic context.</p>
 
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Revision as of 19:29, 1 November 2017


cysPUWA Sulfate Transport System cds


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1274
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 2090
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 3
    Illegal BsaI.rc site found at 3828


Design Notes

[update]

Source

We sourced the nucleotide sequence for cysPUWA from the Escherichia coli MG1655 genome NCBI database (entry NC_000913.3:2539714-2543526). We then sent this sequence to IDT to manufacture as two gBlocks®.

<img src="CysPUWA_cds_NCBI.png" style = "width: 30%">

Figure 1: This image is taken from NCBI and shows the cysPUWA genes in their genomic context.

</img>

References

[update]