Difference between revisions of "Part:BBa K2194003:Design"
Catdunaway (Talk | contribs) |
Catdunaway (Talk | contribs) (→Source) |
||
Line 12: | Line 12: | ||
===Source=== | ===Source=== | ||
− | + | We sourced the nucleotide sequence for <i>cysPUWA</i> from the <i>Escherichia coli</i> MG1655 genome NCBI database (entry NC_000913.3:2539714-2543526). We then sent this sequence to IDT to manufacture as two gBlocks®. | |
+ | |||
+ | <div class = "center caption"> | ||
+ | <img src="https://static.igem.org/mediawiki/parts/4/41/CysPUWA_cds_NCBI.png" style = "width: 60%"> | ||
+ | <p class = "caption"> <b>Figure 1:</b> This image is taken from NCBI and shows the <i>cysPUWA</i> genes in their genomic context.</p> | ||
+ | </img> | ||
===References=== | ===References=== | ||
[update] | [update] |
Revision as of 19:29, 1 November 2017
cysPUWA Sulfate Transport System cds
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 1274
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 2090
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 3
Illegal BsaI.rc site found at 3828
Design Notes
[update]
Source
We sourced the nucleotide sequence for cysPUWA from the Escherichia coli MG1655 genome NCBI database (entry NC_000913.3:2539714-2543526). We then sent this sequence to IDT to manufacture as two gBlocks®.
<img src="" style = "width: 60%">
Figure 1: This image is taken from NCBI and shows the cysPUWA genes in their genomic context.
</img>