Difference between revisions of "Part:BBa K2243015"
 
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<partinfo>BBa_K2243015 short</partinfo>
 
<partinfo>BBa_K2243015 short</partinfo>
  
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Reporter of Bxb1 gp35 integrase.
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Effector for Bxb1 gp35 integrase in Bio-flip-flop.
  
  

Latest revision as of 19:10, 1 November 2017


Bxb1 attB_pTAC_Bxb1 attP

Effector for Bxb1 gp35 integrase in Bio-flip-flop.


Usage

This part is the elementary reporter unit of our Bio-Flip-Flop. It generate another state when the promoter orientation changed along with the recombination of the attB and attP sites.

Biology

J23119 is the consensus sequence of a combinatorial constitutive promoter library family. In nature, Mycobacteriophage Bxb1 integrates its DNA at the attB site of the Mycobacterium smegmatis genome using the viral attP site. We obtained the promoter, attL and attR sites by oligo synthesis.


Design note

We construct this structure by Gibson Assembly.

Characterize

The characterization have not done yet.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 29
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 49
    Illegal BsaI.rc site found at 162