Difference between revisions of "Part:BBa K2384017"
 
Line 6: Line 6:
  
 
<b>Optimization Report</b>
 
<b>Optimization Report</b>
 +
 +
Expression System:Bacillus megaterium
 +
 +
Gene Length:189 (bp)
  
  
Line 13: Line 17:
  
 
<table><tr>
 
<table><tr>
−
<th>[[Image:T--FAFU-CHINA--cspc1.png|thumb|400px|'''Before Codon Adjustment''']]</th>
+
<th>[[Image:T--FAFU-CHINA--cspc1.png|thumb|400px|'''Figure 1:Before Codon Adjustment''']]</th>
 
</tr></table>
 
</tr></table>
  
 
<table><tr>
 
<table><tr>
−
<th>[[Image:T--FAFU-CHINA--cspc2.png|thumb|400px|'''After Codon Adjustment''']]</th>
+
<th>[[Image:T--FAFU-CHINA--cspc2.png|thumb|400px|'''Figure 2:After Codon Adjustment''']]</th>
 
</tr></table>
 
</tr></table>
  
Line 25: Line 29:
  
 
<table><tr>
 
<table><tr>
−
<th>[[Image:T--FAFU-CHINA--cspc3.png|thumb|400px|'''Before Optimization''']]</th>
+
<th>[[Image:T--FAFU-CHINA--cspc3.png|thumb|400px|'''Figure 3:Before Optimization''']]</th>
 
</tr></table>
 
</tr></table>
  
 
<table><tr>
 
<table><tr>
−
<th>[[Image:T--FAFU-CHINA--cspc4.png|thumb|400px|'''After Optimization''']]</th>
+
<th>[[Image:T--FAFU-CHINA--cspc4.png|thumb|400px|'''Figure 4:After Optimization''']]</th>
 
</tr></table>
 
</tr></table>
  
Line 36: Line 40:
  
 
<table><tr>
 
<table><tr>
−
<th>[[Image:T--FAFU-CHINA--cspc5.png|thumb|400px|'''Before Optimization''']]</th>
+
<th>[[Image:T--FAFU-CHINA--cspc5.png|thumb|400px|'''Figure 5:Before Optimization''']]</th>
 
</tr></table>
 
</tr></table>
  
 
<table><tr>
 
<table><tr>
−
<th>[[Image:T--FAFU-CHINA--cspc6.png|thumb|400px|'''After Optimization''']]</th>
+
<th>[[Image:T--FAFU-CHINA--cspc6.png|thumb|400px|'''Figure 6:After Optimization''']]</th>
 
</tr></table>
 
</tr></table>
  
Line 46: Line 50:
  
 
<table><tr>
 
<table><tr>
−
<th>[[Image:T--FAFU-CHINA--cspc7-1.png|thumb|400px|'''After Optimization''']]</th>
+
<th>[[Image:T--FAFU-CHINA--cspc7-1.png|thumb|400px|'''Figure 7:After Optimization''']]</th>
 
</tr></table>
 
</tr></table>
  
 
<table><tr>
 
<table><tr>
−
<th>[[Image:T--FAFU-CHINA--M-CSPC.jpg|thumb|400px|'''On pSB1C3 Digested with
+
<th>[[Image:T--FAFU-CHINA--M-CSPC.jpg|thumb|400px|'''Figure 8:On pSB1C3 Digested with
 
SpeI//
 
SpeI//
 
''']]</th>
 
''']]</th>

Latest revision as of 19:06, 1 November 2017


CspC(For Bacillus megaterium)

This gene is a cold resistant gene from the Arctic cryophile Polaribacter irgensii.It can largely increase the cold resistance of bacterias.

Optimization Report

Expression System:Bacillus megaterium

Gene Length:189 (bp)


1.Codon Used Adjustment The best value is 1 for sequence optimization.


Figure 1:Before Codon Adjustment
Figure 2:After Codon Adjustment


2.Codon Used Distribution Show the relative codon used distribution

Figure 3:Before Optimization
Figure 4:After Optimization

3.GC Content: The comparison of GC content between original sequence and optimized sequence

Figure 5:Before Optimization
Figure 6:After Optimization

4.Restriction Enzyme

Figure 7:After Optimization
Figure 8:On pSB1C3 Digested with SpeI//

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]