Difference between revisions of "Part:BBa K2243010"
 
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Figure 2. The standard testing system used to characterize the recombinases.
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Figure 1. The standard testing system used to characterize the recombinases.
  
 
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Latest revision as of 19:03, 1 November 2017


TP901-1 attB_J23119_TP901-1 attP

The constitutive promoter J23119 is between TP901-1 specific sites and will be inverted by the TP901-1 recombinase, thus leading to the direction of promoter and change of gene expression. This unit can be used as standard reporter of recombinase TP901-1 characterization.

We use this part to be the state switch of the flip-flop. See the link of the bio-flip-flop.

Figure 1. The standard testing system used to characterize the recombinases.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 105
    Illegal NheI site found at 128
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 188
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]