Difference between revisions of "Part:BBa K2443032"
| Line 5: | Line 5: | ||
| − | Ribosome Recycling Factor (RRF) is responsible for recycling ribosomes after completion of protein synthesis. it has been Codon optimized for use in <i>Escherichia coli</i> and contains a C-terminal hexahistidine tag. Under the regulation of T7 Promoter, RBS and double terminator. | + | Ribosome Recycling Factor (RRF) is responsible for recycling ribosomes after completion of protein synthesis. it has been Codon optimized for use in <i>Escherichia coli</i> and contains a C-terminal hexahistidine tag. Under the regulation of T7 Promoter, RBS and double terminator. The expected size of RRF is 22 kDa, which closely corresponds to the band in lane 9 of the overexpression gel below. Protein was overexpressed from BL21DE3 gold containing pJET vector with the RRF coding sequence. Protein overexpression was performed according to the protocol on the Lethbridge 2017 iGEM wiki, and notebook (https://static.igem.org/mediawiki/2017/2/2c/Protein_Overexpression_.pdf and https://static.igem.org/mediawiki/2017/0/0d/October_.pdf) |
| + | https://static.igem.org/mediawiki/parts/b/bf/Lethbridge_Oct_22_gel.png | ||
<!-- Add more about the biology of this part here | <!-- Add more about the biology of this part here | ||
Latest revision as of 19:00, 1 November 2017
RRF optimized for expression in E. coli
Ribosome Recycling Factor (RRF) is responsible for recycling ribosomes after completion of protein synthesis. it has been Codon optimized for use in Escherichia coli and contains a C-terminal hexahistidine tag. Under the regulation of T7 Promoter, RBS and double terminator. The expected size of RRF is 22 kDa, which closely corresponds to the band in lane 9 of the overexpression gel below. Protein was overexpressed from BL21DE3 gold containing pJET vector with the RRF coding sequence. Protein overexpression was performed according to the protocol on the Lethbridge 2017 iGEM wiki, and notebook (https://static.igem.org/mediawiki/2017/2/2c/Protein_Overexpression_.pdf and https://static.igem.org/mediawiki/2017/0/0d/October_.pdf)
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]