Difference between revisions of "Part:BBa K2384003"
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<b>Optimization Report</b>
 
<b>Optimization Report</b>
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 +
Gene Name:DSBA-MBP(Pb)
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 +
Expression System:Bacillus megaterium
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 +
Gene Length:963 (bp)
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1.Codon Used Adjustment
 
1.Codon Used Adjustment
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<table><tr>
 
<table><tr>
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<th>[[Image:T--FAFU-CHINA--DSBA-MBP(Pb)1.png|thumb|400px|'''Before Codon Adjustment''']]</th>
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<th>[[Image:T--FAFU-CHINA--DSBA-MBP(Pb)1.png|thumb|400px|'''Figure 1:Before Codon Adjustment''']]</th>
 
</tr></table>
 
</tr></table>
  
 
<table><tr>
 
<table><tr>
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<th>[[Image:T--FAFU-CHINA--DSBA-MBP(Pb)2.png|thumb|400px|'''After Codon Adjustment''']]</th>
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<th>[[Image:T--FAFU-CHINA--DSBA-MBP(Pb)2.png|thumb|400px|'''Figure 2:After Codon Adjustment''']]</th>
 
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<table><tr>
 
<table><tr>
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<th>[[Image:T--FAFU-CHINA--DSBA-MBP(Pb)3.png|thumb|400px|'''Before Optimization''']]</th>
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<th>[[Image:T--FAFU-CHINA--DSBA-MBP(Pb)3.png|thumb|400px|'''Figure 3:Before Optimization''']]</th>
 
</tr></table>
 
</tr></table>
  
 
<table><tr>
 
<table><tr>
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<th>[[Image:T--FAFU-CHINA--DSBA-MBP(Pb)4.png|thumb|400px|'''After Optimization''']]</th>
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<th>[[Image:T--FAFU-CHINA--DSBA-MBP(Pb)4.png|thumb|400px|'''Figure 4:After Optimization''']]</th>
 
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<table><tr>
 
<table><tr>
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<th>[[Image:T--FAFU-CHINA--DSBA-MBP(Pb)5.png|thumb|400px|'''Before Optimization''']]</th>
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<th>[[Image:T--FAFU-CHINA--DSBA-MBP(Pb)5.png|thumb|400px|'''Figure 5:Before Optimization''']]</th>
 
</tr></table>
 
</tr></table>
  
 
<table><tr>
 
<table><tr>
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<th>[[Image:T--FAFU-CHINA--DSBA-MBP(Pb)(Pb)6.png|thumb|400px|'''After Optimization''']]</th>
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<th>[[Image:T--FAFU-CHINA--DSBA-MBP(Pb)(Pb)6.png|thumb|400px|'''Figure 6:After Optimization''']]</th>
 
</tr></table>
 
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<table><tr>
 
<table><tr>
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<th>[[Image:T--FAFU-CHINA--DsbA-MBP123.jpg|thumb|400px|'''The expression of DsbA+MBP(Pb) detected using SDS-PAGE ''']]</th>
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<th>[[Image:T--FAFU-CHINA--DsbA-MBP123.jpg|thumb|400px|'''Figure 7:The expression of DsbA+MBP(Pb) detected using SDS-PAGE ''']]</th>
 
</tr></table>
 
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<table><tr>
 
<table><tr>
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<th>[[Image:T--FAFU-CHINA--M-DSBA-MBP(Pb).png|thumb|400px|'''Plasmid  digested by EcoRI-PstI ''']]</th>
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<th>[[Image:T--FAFU-CHINA--M-DSBA-MBP(Pb).png|thumb|400px|'''Figure 8:Plasmid  digested by EcoRI-PstI ''']]</th>
 
</tr></table>
 
</tr></table>
  

Revision as of 18:56, 1 November 2017


DsbA+MBP(Pb)

the periplasm display protein Dsba-MBP, which binding the lead of the enviroment. Disulfide bond formation protein A (DsbA) is a powerful oxidative protein of the thioredoxin family. DsbA can help protein to fold correctly both in vivo and in vitro 。The C-terminal fusion protein of the maltose-binding protein can be efficiently and rapidly output into the periplasm as a key component of the expression of the metal-binding peptide of the cyclin.

Our sequence optimization is based on the sequence provided by Peking University's IGEM Wiki in 2010.It is more suitable for expression in Bacillus


Usage and Biology

Optimization Report

Gene Name:DSBA-MBP(Pb)

Expression System:Bacillus megaterium

Gene Length:963 (bp)


1.Codon Used Adjustment The best value is 1 for sequence optimization.


Figure 1:Before Codon Adjustment
Figure 2:After Codon Adjustment


2.Codon Used Distribution Show the relative codon used distribution

Figure 3:Before Optimization
Figure 4:After Optimization

3.GC Content: The comparison of GC content between original sequence and optimized sequence

Figure 5:Before Optimization
Figure 6:After Optimization
Figure 7:The expression of DsbA+MBP(Pb) detected using SDS-PAGE
Figure 8:Plasmid digested by EcoRI-PstI


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 255
    Illegal BglII site found at 267
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]