Difference between revisions of "Part:BBa K2295002"
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Hypoxia induced factors (HIFs) are transcription factors responding to decreased oxigen levels in the cellular environment. | Hypoxia induced factors (HIFs) are transcription factors responding to decreased oxigen levels in the cellular environment. | ||
Hypoxia-inducible factor 1-alpha (HIF1A) is a protein constantly expressed in mammalian cells. Under normoxic conditions HIF1A is hydroxylated and is marked by the E3 ubiquitin ligase which leads to the degradation by the proteasome. However, in hypoxic conditions HIF1A is stabilized and can heterodimerize with HIF1B. Also HIF1A transcription is often significantly upregulated under hypoxic conditions. Under hypoxic conditions HIF1 can then bind to hypoxia response elements (HREs) (<partinfo>BBa_K2295003</partinfo>) in the nucleus and lead to the expression of the gene of interest (Ziello et al., 2007). | Hypoxia-inducible factor 1-alpha (HIF1A) is a protein constantly expressed in mammalian cells. Under normoxic conditions HIF1A is hydroxylated and is marked by the E3 ubiquitin ligase which leads to the degradation by the proteasome. However, in hypoxic conditions HIF1A is stabilized and can heterodimerize with HIF1B. Also HIF1A transcription is often significantly upregulated under hypoxic conditions. Under hypoxic conditions HIF1 can then bind to hypoxia response elements (HREs) (<partinfo>BBa_K2295003</partinfo>) in the nucleus and lead to the expression of the gene of interest (Ziello et al., 2007). | ||
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===Western Blot against HIF1A=== | ===Western Blot against HIF1A=== |
Revision as of 18:51, 1 November 2017
HIF1A (Hypoxia-inducible factor one alpha)
Mechanism
Hypoxia induced factors (HIFs) are transcription factors responding to decreased oxigen levels in the cellular environment. Hypoxia-inducible factor 1-alpha (HIF1A) is a protein constantly expressed in mammalian cells. Under normoxic conditions HIF1A is hydroxylated and is marked by the E3 ubiquitin ligase which leads to the degradation by the proteasome. However, in hypoxic conditions HIF1A is stabilized and can heterodimerize with HIF1B. Also HIF1A transcription is often significantly upregulated under hypoxic conditions. Under hypoxic conditions HIF1 can then bind to hypoxia response elements (HREs) (BBa_K2295003) in the nucleus and lead to the expression of the gene of interest (Ziello et al., 2007).
Western Blot against HIF1A
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 79
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 10
Illegal AgeI site found at 1086 - 1000COMPATIBLE WITH RFC[1000]