Difference between revisions of "Part:BBa K2515002"

Revision as of 18:46, 1 November 2017

gRNA expression vector for use with Cas9 or dCas9

This is the pSB1C3-gRNA vector designed and used by Team Bulgaria 2017. Our gRNA cloning cassette consist of four functional regions. First comes the constitutive promoter J23119 with defined transcription start. It is fused to the second base pairing region which itself is surrounded by two unique Eco31I sites. Next comes a gRNA scaffold region followed by a S. pyogenes terminator.

For more details and protocols - see Design page.
For a functional test of cloned gRNAs - see Experience page.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 8
Illegal NheI site found at 31
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI site found at 64
Illegal BsaI.rc site found at 38

@@ Line 4: / Line 4: @@
 <p align="justify"><br />This is the pSB1C3-gRNA vector designed and used by Team Bulgaria 2017. Our gRNA cloning cassette consist of four functional regions. First comes the constitutive promoter J23119 with defined transcription start. It is fused to the second base pairing region which itself is surrounded by two unique Eco31I sites. Next comes a gRNA scaffold region followed by a <i>S. pyogenes</i> terminator.<br /><br /></p>
+<b>For more details and protocols - see <i>Design</i> page.<br />
+For a functional test of cloned gRNAs - see <i>Experience</i> page.</b><br /><br />
 <!-- Add more about the biology of this part here