Difference between revisions of "Part:BBa K2505030:Design"
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The DNA sequences of <i>traI</i> (K34G) is optimized for expressing in <i>E. coli</i> considering the codon usage. | The DNA sequences of <i>traI</i> (K34G) is optimized for expressing in <i>E. coli</i> considering the codon usage. | ||
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Mutation was newly introduced on the <partinfo>BBa_K553001</partinfo> by PCR. | Mutation was newly introduced on the <partinfo>BBa_K553001</partinfo> by PCR. | ||
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===References=== | ===References=== | ||
Revision as of 18:44, 1 November 2017
Ptet-rbs-traI (K34G)-tt
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
The gene traI(K34G) is derived from Agrobacterium tumefaciens and encode a enzyme necessary for synthesizing Quorum Sensing signaling molecules ([N-]acyl-homoserine lactones, AHLs), 3OC8 HSL (hereafter C8), in E. coli. This part constitutively produces C8. We introduced point mutation to traI gene and the productivity of C8 was improved by approximately 3-fold. We introduced this part to E. coli then E. coli could produced enough C8 to induce transcription of human cells.
The mutation was introduced wild type traI(BBa_K553001).
The DNA sequences of traI (K34G) is optimized for expressing in E. coli considering the codon usage.
Source
Mutation was newly introduced on the BBa_K553001 by PCR.
References
[1] Pavan Kumar Reddy Kambam, Daniel J. Sayut, Yan Niu, Dawn T. Eriksen, Lianhong Sun (2008) Directed evolution of LuxI for enhanced OHHL production. Biotechnology and Bioengineering Volume 101, Issue 2 1 October 2008 Pages 263-272
[2] MATTHEW R. PARSEK, DALE L. VAL, BRIAN L. HANZELKA, JOHN E. CRONAN, E. P. GREENBERG (1999) Acyl homoserine-lactone quorum-sensing signal generation. Proc. Natl. Acad. Sci. USA Vol. 96, pp. 4360-4365, April 1999 Biochemistry