Difference between revisions of "Part:BBa K2505010:Design"
 
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===Design Notes===
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==Design Notes==
 
The gene <i>traI</i> is derived from <i>Agrobacterium tumefaciens</i> and encode a enzyme necessary for synthesizing Quorum Sensing signaling molecules ([N-]acyl-homoserine lactones, AHLs), 3OC8 HSL (hereafter C8), in <i>E. coli</i>.  
 
The gene <i>traI</i> is derived from <i>Agrobacterium tumefaciens</i> and encode a enzyme necessary for synthesizing Quorum Sensing signaling molecules ([N-]acyl-homoserine lactones, AHLs), 3OC8 HSL (hereafter C8), in <i>E. coli</i>.  
 
This part constitutively produces C8.  
 
This part constitutively produces C8.  

Latest revision as of 18:43, 1 November 2017


Ptet-rbs-traI-tt


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 465
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The gene traI is derived from Agrobacterium tumefaciens and encode a enzyme necessary for synthesizing Quorum Sensing signaling molecules ([N-]acyl-homoserine lactones, AHLs), 3OC8 HSL (hereafter C8), in E. coli. This part constitutively produces C8.

The DNA sequences of traI (K34G) is optimized for expressing in E. coli considering the codon usage.


Source

TraI was derived from agrobacterium.

References

[1]. http://2014.igem.org/Team:ETH_Zurich/modeling/qs

[2]. Elise R. Morton and Clay Fuqua (2012) UNIT 3D.1 Laboratory Maintenance of Agrobacterium. Curr Protoc Microbiol. 2012 Feb; CHAPTER: Unit3D.1.

[3]. http://2007.igem.org/wiki/index.php/Chiba/Quorum_Sensing