Difference between revisions of "Part:BBa K2505008:Design"
 
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===References===
 
===References===
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Conserved cis-Acting Promoter Elements Are Required for Density-Dependent Transcription of Agrobacterium tumefaciens Conjugal Transfer Genes, 1995 CLAY FUQUA et.al
+
C Fuqua, S C Winans. (1996) Conserved cis-acting promoter elements are required for density-dependent transcription of Agrobacterium tumefaciens conjugal transfer genes. J Bacteriol. 178: 435–440.

Latest revision as of 18:38, 1 November 2017


Ptet-rbs-traR-tt


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The traR gene is derived from A. Tumefaciens and the codon of this part is optimized for E. coli in terms of codon usage. TraR protein recieves Quorum Sensing signaling molecules ([N-]acyl-homoserine lactones, AHLs), 3OC8 HSL (hereafter C8), leading to the activaion of the trascription.

Source

This part is derived from Agrobacterium.

References

C Fuqua, S C Winans. (1996) Conserved cis-acting promoter elements are required for density-dependent transcription of Agrobacterium tumefaciens conjugal transfer genes. J Bacteriol. 178: 435–440.