Difference between revisions of "Part:BBa K2324009"

Revision as of 18:33, 1 November 2017

pRha_FimH_1SynMT

This part produces a FimH adhesin protein fused with a metallothionein domain from Synechococcus elongatus at the first amino acid position. The coding sequence (BBa_K2324004) is under the control of a rhamnose-inducible promoter, with a B0034 RBS and a B0015 terminator. The part, when induced, produces a FimH+metal binding protein that should initiate pilus biosynthesis when co-transformed with a plasmid containing the fim operon .

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal NgoMIV site found at 1145
Illegal AgeI site found at 572
1000
COMPATIBLE WITH RFC[1000]

@@ Line 3: / Line 3: @@
 <partinfo>BBa_K2324009 short</partinfo>
-This part produces a FimH adhesin protein fused with a metallothionein domain at the first amino acid position. The coding sequence (BBa_K2324004) is under the control of a rhamnose-inducible promoter, with a B0034 RBS and a B0015 terminator. The part, when induced, produces a FimH+metal binding protein that should initiate pilus biosynthesis when co-transformed with a plasmid containing the <i>fim operon</i> .
+This part produces a FimH adhesin protein fused with a metallothionein domain from <i>Synechococcus elongatus</i> at the first amino acid position. The coding sequence (BBa_K2324004) is under the control of a rhamnose-inducible promoter, with a B0034 RBS and a B0015 terminator. The part, when induced, produces a FimH+metal binding protein that should initiate pilus biosynthesis when co-transformed with a plasmid containing the <i>fim operon</i> .
-<!-- Add more about the biology of this part here
-===Usage and Biology===
-<!-- -->
 <span class='h3bb'>Sequence and Features</span>
 <partinfo>BBa_K2324009 SequenceAndFeatures</partinfo>