Difference between revisions of "Part:BBa K2384002"
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It is highly expected that when fused with N-terminal fragment of Outer membrane protein A (OmpA), the mercury metal binding peptide(MBP)will be translocated and displayed on the surface of bacteria, which will contribute a lot to increase the mental binding capacity when combined will other collection parts. | It is highly expected that when fused with N-terminal fragment of Outer membrane protein A (OmpA), the mercury metal binding peptide(MBP)will be translocated and displayed on the surface of bacteria, which will contribute a lot to increase the mental binding capacity when combined will other collection parts. | ||
In the Lpp-Omp display system with potential for displaying macromolecular proteins,it contains signal peptide and the first nine amino acids of Lpp and forty-sixth to 159th amino acids of OmpA.The former contains all the information that carries the protein to the outer membrane and anchors it to the inside of the outer membrane,and the latter can anchor the target fusion protein to the outside of the membrane.The MBP that follows it can be expose. | In the Lpp-Omp display system with potential for displaying macromolecular proteins,it contains signal peptide and the first nine amino acids of Lpp and forty-sixth to 159th amino acids of OmpA.The former contains all the information that carries the protein to the outer membrane and anchors it to the inside of the outer membrane,and the latter can anchor the target fusion protein to the outside of the membrane.The MBP that follows it can be expose. | ||
| − | Our sequence optimization is based on the sequence provided by Peking University's IGEM | + | Our sequence optimization is based on the sequence provided by Peking University's IGEM Wiki in 2010.It is more suitable for expression in Bacillus megaterium. |
<b>Optimization Report</b> | <b>Optimization Report</b> | ||
Revision as of 18:32, 1 November 2017
Lpp-OmpA-MBP(Hg)
It is highly expected that when fused with N-terminal fragment of Outer membrane protein A (OmpA), the mercury metal binding peptide(MBP)will be translocated and displayed on the surface of bacteria, which will contribute a lot to increase the mental binding capacity when combined will other collection parts. In the Lpp-Omp display system with potential for displaying macromolecular proteins,it contains signal peptide and the first nine amino acids of Lpp and forty-sixth to 159th amino acids of OmpA.The former contains all the information that carries the protein to the outer membrane and anchors it to the inside of the outer membrane,and the latter can anchor the target fusion protein to the outside of the membrane.The MBP that follows it can be expose. Our sequence optimization is based on the sequence provided by Peking University's IGEM Wiki in 2010.It is more suitable for expression in Bacillus megaterium.
Optimization Report
1.Codon Used Adjustment The best value is 1 for sequence optimization.
1.png)
2.png)
2.Codon Used Distribution
Show the relative codon used distribution
3.png)
4.png)
3.GC Content: The comparison of GC content between original sequence and optimized sequence
5.png)
6.png)
.png)
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 513
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]