Difference between revisions of "Part:BBa K2324007"

Revision as of 18:30, 1 November 2017

pRha_FimH_1sfGFP

This part produces a FimH adhesin protein fused with GFP at the 1st amino acid position after the signal peptide has been cleaved. The coding sequence is under the control of a rhamnose-inducible promoter, with a B0034 RBS and a B0015 terminator. The part, when induced, produces a fluorescent FimH protein that should initiate pilus biosynthesis when co-transformed with a plasmid containing the fim operon .

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

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 <partinfo>BBa_K2324007 short</partinfo>
-This part synthesises a FimH adhesin protein fused with GFP at the 1st amino acid position after the signal peptide has been cleaved. The coding sequence is under the control of a rhamnose-inducible promoter, with a B0034 RBS and a B0015 terminator. The part, when induced, produces a fluorescent FimH protein that should involve itself in pilus biosynthesis when co-transformed with a plasmid containing the fim operon .
+This part produces a FimH adhesin protein fused with GFP at the 1st amino acid position after the signal peptide has been cleaved. The coding sequence is under the control of a rhamnose-inducible promoter, with a B0034 RBS and a B0015 terminator. The part, when induced, produces a fluorescent FimH protein that should initiate pilus biosynthesis when co-transformed with a plasmid containing the <i>fim operon</i> .
 <!-- Add more about the biology of this part here