Difference between revisions of "Part:BBa K2324006"
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This part produces a FimH adhesin protein fused with sfGFP at its 225th amino acid residue. The coding sequence | This part produces a FimH adhesin protein fused with sfGFP at its 225th amino acid residue. The coding sequence | ||
is under the control of a rhamnose-inducible promoter, with a B0034 RBS and a B0015 terminator. The part, when induced, | is under the control of a rhamnose-inducible promoter, with a B0034 RBS and a B0015 terminator. The part, when induced, | ||
| − | produces a fluorescent FimH protein that should | + | produces a fluorescent FimH protein that should initiate pilus biosynthesis when co-transformed with a plasmid |
containing the <i> fim operon </i>. | containing the <i> fim operon </i>. | ||
Revision as of 18:29, 1 November 2017
pRha_FimH_225sfGFP
This part produces a FimH adhesin protein fused with sfGFP at its 225th amino acid residue. The coding sequence is under the control of a rhamnose-inducible promoter, with a B0034 RBS and a B0015 terminator. The part, when induced, produces a fluorescent FimH protein that should initiate pilus biosynthesis when co-transformed with a plasmid containing the fim operon .
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 451
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]