Difference between revisions of "Part:BBa K2243010"
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<partinfo>BBa_K2243010 short</partinfo>
 
<partinfo>BBa_K2243010 short</partinfo>
  
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The constitutive promoter J23119 is between Bxb1 gp35 specific sites and will be inverted by the Bxb1 gp35 recombinase, thus leading to the direction of promoter and change of gene expression. This unit can be used as standard reporter of recombinase bxb1 gp35 characterization.  
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The constitutive promoter J23119 is between TP901-1 specific sites and will be inverted by the TP901-1 recombinase, thus leading to the direction of promoter and change of gene expression. This unit can be used as standard reporter of recombinase TP901-1 characterization.  
  
 
We use this part to be the state switch of the flip-flop. See the link of the bio-flip-flop.
 
We use this part to be the state switch of the flip-flop. See the link of the bio-flip-flop.

Revision as of 18:28, 1 November 2017


TP901-1 attB_J23119_TP901-1 attP

The constitutive promoter J23119 is between TP901-1 specific sites and will be inverted by the TP901-1 recombinase, thus leading to the direction of promoter and change of gene expression. This unit can be used as standard reporter of recombinase TP901-1 characterization.

We use this part to be the state switch of the flip-flop. See the link of the bio-flip-flop.

Figure 2. The standard testing system used to characterize the recombinases.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 105
    Illegal NheI site found at 128
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 188
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]