Difference between revisions of "Part:BBa K2136013"
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Constitutive promoter for use in Chlamydomonas reinhardtii based on the fusion of DNA sequences upstream of Heat Shock Protein 70A (nucleotides 1-275) and rbcS2 (nucleotides 294-474) genes.
 
Constitutive promoter for use in Chlamydomonas reinhardtii based on the fusion of DNA sequences upstream of Heat Shock Protein 70A (nucleotides 1-275) and rbcS2 (nucleotides 294-474) genes.
 
This part also includes a 5'-UTR derived from the rbcS2 gene.  
 
This part also includes a 5'-UTR derived from the rbcS2 gene.  
 +
 +
  
 
<b>Improved part in 2017</b>
 
<b>Improved part in 2017</b>

Revision as of 18:21, 1 November 2017


hsp70 + rbsc2 synthetic constitutive promoter for Chlamydomonas

AR1-Rbcs2

Constitutive promoter for use in Chlamydomonas reinhardtii based on the fusion of DNA sequences upstream of Heat Shock Protein 70A (nucleotides 1-275) and rbcS2 (nucleotides 294-474) genes. This part also includes a 5'-UTR derived from the rbcS2 gene.


Improved part in 2017

NU_Kazakhstan 2017 team made an improved version of this promoter by inserting high-efficiency separator between two promoters and added the first intron of RBCS2 gene. Link: https://parts.igem.org/Part:BBa_K2516001


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 264
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]