Difference between revisions of "Part:BBa K2348011"

Revision as of 18:17, 1 November 2017

asr promoter + mCardinal
This part actually is a composite part containing the asr promoter BBa_K2348001 and mCardinal BBa_K2348002. Additionally, between promoter and cardinal an additional RBS, a TEV-site and a f-degron are inserted. Also, mCardinal is 6xHis-taged and the part ends with a T7-terminator. Because the part does not work when build as Biobrick due to the Biobrick scar this part is listed as basic part.
TEV-site and F-degron allow a fast and controllable degradation of mNeonGreen wich, in combination with the fast maturation time of mNeonGreen, lead to a fast colour change when used together with our other pH-sensitive part BBa_K2348012

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI.rc site found at 639
Illegal BsaI.rc site found at 828

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 <partinfo>BBa_K2348011 short</partinfo><br>
 This part actually is a composite part containing the asr promoter [https://parts.igem.org/Part:BBa_K2348001 BBa_K2348001] and mCardinal [https://parts.igem.org/Part:BBa_K2348002 BBa_K2348002]. Additionally, between promoter and cardinal an additional RBS, a TEV-site and a f-degron are inserted. Also, mCardinal is 6xHis-taged and the part ends with a T7-terminator. Because the part does not work when build as Biobrick due to the Biobrick scar this part is listed as basic part.<br>
-TEV-site and f-degron allow a fast and controllable degradation of proteins and in combination with the fast maturation times of our fluorescence proteins, allow a fast colour change. This enables shorter time spans between signals when controlling the robot’s movement. Due to a change of our reactor design this feature is no longer needed because the pH is only changed in a small amount of media which is discarded after measurement.
+TEV-site and F-degron allow a fast and controllable degradation of mNeonGreen wich, in combination with the fast maturation time of mNeonGreen, lead to a fast colour change when used together with our other pH-sensitive part [https://parts.igem.org/Part:BBa_K2348012 BBa_K2348012]
-The 6xHis-tag allows detection of mNeonGreen on protein level and the T7-terminator stops translation. The part itself was created using extension PCR and overlap PCR.