Difference between revisions of "Part:BBa K2348011"
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<partinfo>BBa_K2348011 short</partinfo><br> | <partinfo>BBa_K2348011 short</partinfo><br> | ||
| − | This part actually is a composite part containing the asr promoter [https://parts.igem.org/Part:BBa_K2348001 BBa_K2348001] and mCardinal [https://parts.igem.org/Part:BBa_K2348002 BBa_K2348002]. Additionally, between promoter and cardinal an additional RBS, a TEV-site and a f-degron are inserted. Also, mCardinal is 6xHis-taged and the part ends with a T7-terminator. Because the part does not work when build as Biobrick due to the Biobrick scar this part is listed as basic part. | + | This part actually is a composite part containing the asr promoter [https://parts.igem.org/Part:BBa_K2348001 BBa_K2348001] and mCardinal [https://parts.igem.org/Part:BBa_K2348002 BBa_K2348002]. Additionally, between promoter and cardinal an additional RBS, a TEV-site and a f-degron are inserted. Also, mCardinal is 6xHis-taged and the part ends with a T7-terminator. Because the part does not work when build as Biobrick due to the Biobrick scar this part is listed as basic part.<br> |
TEV-site and f-degron allow a fast and controllable degradation of proteins and in combination with the fast maturation times of our fluorescence proteins, allow a fast colour change. This enables shorter time spans between signals when controlling the robot’s movement. Due to a change of our reactor design this feature is no longer needed because the pH is only changed in a small amount of media which is discarded after measurement. | TEV-site and f-degron allow a fast and controllable degradation of proteins and in combination with the fast maturation times of our fluorescence proteins, allow a fast colour change. This enables shorter time spans between signals when controlling the robot’s movement. Due to a change of our reactor design this feature is no longer needed because the pH is only changed in a small amount of media which is discarded after measurement. | ||
The 6xHis-tag allows detection of mNeonGreen on protein level and the T7-terminator stops translation. The part itself was created using extension PCR and overlap PCR. | The 6xHis-tag allows detection of mNeonGreen on protein level and the T7-terminator stops translation. The part itself was created using extension PCR and overlap PCR. | ||
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| − | <span class='h3bb'>Sequence and Features</span> | + | <span class='h3bb'><b>Sequence and Features<b></span><br> |
| + | https://static.igem.org/mediawiki/2017/thumb/9/96/Asr_construct_Graz.jpeg/800px-Asr_construct_Graz.jpeg | ||
<partinfo>BBa_K2348011 SequenceAndFeatures</partinfo> | <partinfo>BBa_K2348011 SequenceAndFeatures</partinfo> | ||
Revision as of 18:16, 1 November 2017
asr promoter + mCardinal
This part actually is a composite part containing the asr promoter BBa_K2348001 and mCardinal BBa_K2348002. Additionally, between promoter and cardinal an additional RBS, a TEV-site and a f-degron are inserted. Also, mCardinal is 6xHis-taged and the part ends with a T7-terminator. Because the part does not work when build as Biobrick due to the Biobrick scar this part is listed as basic part.
TEV-site and f-degron allow a fast and controllable degradation of proteins and in combination with the fast maturation times of our fluorescence proteins, allow a fast colour change. This enables shorter time spans between signals when controlling the robot’s movement. Due to a change of our reactor design this feature is no longer needed because the pH is only changed in a small amount of media which is discarded after measurement.
The 6xHis-tag allows detection of mNeonGreen on protein level and the T7-terminator stops translation. The part itself was created using extension PCR and overlap PCR.
Sequence and Features<b></span>
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 639
Illegal BsaI.rc site found at 828