Difference between revisions of "Part:BBa I763032:Experience"
Line 1: Line 1:
 
__NOTOC__
 
__NOTOC__
  
−
As expected, we observe that bacteria transformed with BBa I763032 are no fluorescent in LB medium untill they reach the growth stationary phase. When all the glucose is depleted bacteria are fluorescent due to lack of the catabolite repression from cAMP- CAP complex.
+
As expected, we observed that bacteria transformed with BBa I763032 are no fluorescent in LB medium untill they reached the growth stationary phase. When all the glucose was depleted bacteria were fluorescent due to lack of the catabolite repression from cAMP- CAP complex.
  
 
We first cloned the part inside a high copy number plasmid (pSB1AK3). When we checked part lenght with a gel electrophoresis that was ok. Anyway, when we checked for fluorescence we observed that just some bacteria were fluorescent. We grew bacteria in M9 + lactose + IPTG (2 mM),but always we saw that not all of them were fluorescent.
 
We first cloned the part inside a high copy number plasmid (pSB1AK3). When we checked part lenght with a gel electrophoresis that was ok. Anyway, when we checked for fluorescence we observed that just some bacteria were fluorescent. We grew bacteria in M9 + lactose + IPTG (2 mM),but always we saw that not all of them were fluorescent.

Revision as of 13:29, 26 October 2007


As expected, we observed that bacteria transformed with BBa I763032 are no fluorescent in LB medium untill they reached the growth stationary phase. When all the glucose was depleted bacteria were fluorescent due to lack of the catabolite repression from cAMP- CAP complex.

We first cloned the part inside a high copy number plasmid (pSB1AK3). When we checked part lenght with a gel electrophoresis that was ok. Anyway, when we checked for fluorescence we observed that just some bacteria were fluorescent. We grew bacteria in M9 + lactose + IPTG (2 mM),but always we saw that not all of them were fluorescent.

So, we tried tried the same cloning in a low copy number plasmid (pSB4A3), thinking that it could better with a lower amount of cI repressor, but we obtained the same result.


Applications of BBa_I763032

User Reviews

UNIQafce4ebe8a499670-partinfo-00000000-QINU UNIQafce4ebe8a499670-partinfo-00000001-QINU