Difference between revisions of "Part:BBa K2384018"

Revision as of 18:00, 1 November 2017

Repressor gene xylR+Strong PxylA promoter+RBS+DsbA-MBP(Pb)+His-tag

Xylose-inducible expression system: strong PxylA promoter and repressor gene xylR; after xylose addition, the repressor is released from the PxylA that activates transcription initiation.(BBa_K733002) They have been designed for high expression in B. megaterium.The periplasm display protein Dsba-MBP, which binding the lead of the enviroment. Disulfide bond formation protein A (DsbA) is a powerful oxidative protein of the thioredoxin family. DsbA can help protein to fold correctly both in vivo and in vitro 。The C-terminal fusion protein of the maltose-binding protein can be efficiently and rapidly output into the periplasm as a key component of the expression of the metal-binding peptide of the cyclin. This year our parts were doing codon optimisation but without terminator codon.

Usage and Biology

Optimization Report

1.Codon Used Adjustment The best value is 1 for sequence optimization.

Before Codon Adjustment

After Codon Adjustment

2.Codon Used Distribution Show the relative codon used distribution

Before Optimization

After Optimization

3.GC Content: The comparison of GC content between original sequence and optimized sequence

Before Optimization

After Optimization

The expression of DsbA+MBP(Pb) detected using SDS-PAGE

Plasmid digested by EcoRI-PstI

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Sequence and Features