Difference between revisions of "Part:BBa K2384018"
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<th>[[Image:T--FAFU-CHINA--Lpp-OmpA-MBP(Pb)1.png|thumb|400px|'''Before Codon Adjustment''']]</th>
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<th>[[Image:T--FAFU-CHINA--DSBA-MBP(Pb)1.png|thumb|400px|'''Before Codon Adjustment''']]</th>
 
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<th>[[Image:T--FAFU-CHINA--Lpp-OmpA-MBP(Pb)2.png|thumb|400px|'''After Codon Adjustment''']]</th>
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<th>[[Image:T--FAFU-CHINA--Lpp-OmpA-MBP(Pb)3.png|thumb|400px|'''Before Optimization''']]</th>
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<th>[[Image:T--FAFU-CHINA--DSBA-MBP(Pb)3.png|thumb|400px|'''Before Optimization''']]</th>
 
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<th>[[Image:T--FAFU-CHINA--Lpp-OmpA-MBP(Pb)4.png|thumb|400px|'''After Optimization''']]</th>
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<th>[[Image:T--FAFU-CHINA--Lpp-OmpA-MBP(Pb)5.png|thumb|400px|'''Before Optimization''']]</th>
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<th>[[Image:T--FAFU-CHINA--DSBA-MBP(Pb)5.png|thumb|400px|'''Before Optimization''']]</th>
 
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<th>[[Image:T--FAFU-CHINA--Lpp-OmpA-MBP(Pb)6.png|thumb|400px|'''After Optimization''']]</th>
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<th>[[Image:T--FAFU-CHINA--DSBA-MBP(Pb)(Pb)6.png|thumb|400px|'''After Optimization''']]</th>
 
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<th>[[Image:T--FAFU-CHINA--Lpp-OmpA-MBP SDS PAGE.png|thumb|400px|'''The expression of Lpp-OmpA-MBP detected using SDS-PAGE ''']]</th>
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<th>[[Image:T--FAFU-CHINA--DsbA-MBP123.jpg|thumb|400px|'''The expression of DsbA+MBP(Pb) detected using SDS-PAGE ''']]</th>
 
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<th>[[Image:T--FAFU-CHINA--M-Lpp-OmpA+MBP(Pb).png|thumb|400px|'''Plasmid digested by PvuII''']]</th>
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<th>[[Image:T--FAFU-CHINA--M-DSBA-MBP(Pb).png|thumb|400px|'''Plasmid digested by EcoRI-PstI ''']]</th>
 
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Revision as of 17:59, 1 November 2017


Repressor gene xylR+Strong PxylA promoter+RBS+DsbA-MBP(Pb)+His-tag

Xylose-inducible expression system: strong PxylA promoter and repressor gene xylR; after xylose addition, the repressor is released from the PxylA that activates transcription initiation.(BBa_K733002) They have been designed for high expression in B. megaterium.The periplasm display protein Dsba-MBP, which binding the lead of the enviroment. Disulfide bond formation protein A (DsbA) is a powerful oxidative protein of the thioredoxin family. DsbA can help protein to fold correctly both in vivo and in vitro 。The C-terminal fusion protein of the maltose-binding protein can be efficiently and rapidly output into the periplasm as a key component of the expression of the metal-binding peptide of the cyclin. This year our parts were doing codon optimisation but without terminator codon.


Usage and Biology

Optimization Report

1.Codon Used Adjustment The best value is 1 for sequence optimization.


Before Codon Adjustment
After Codon Adjustment


2.Codon Used Distribution Show the relative codon used distribution

Before Optimization
After Optimization

3.GC Content: The comparison of GC content between original sequence and optimized sequence

Before Optimization
After Optimization
The expression of DsbA+MBP(Pb) detected using SDS-PAGE
Plasmid digested by EcoRI-PstI


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1668
    Illegal BglII site found at 1680
    Illegal XhoI site found at 847
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]