Difference between revisions of "Part:BBa K2384015"

 
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===Usage and Biology===
 
===Usage and Biology===
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<b>Optimization Report</b>
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1.Codon Used Adjustment
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The best value is 1 for sequence optimization.
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<table><tr>
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<th>[[Image:T--FAFU-CHINA--Lpp-OmpA-MBP(Pb)1.png|thumb|400px|'''Before Codon Adjustment''']]</th>
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</tr></table>
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<table><tr>
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<th>[[Image:T--FAFU-CHINA--Lpp-OmpA-MBP(Pb)2.png|thumb|400px|'''After Codon Adjustment''']]</th>
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</tr></table>
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2.Codon Used Distribution
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Show the relative codon used distribution
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<table><tr>
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<th>[[Image:T--FAFU-CHINA--Lpp-OmpA-MBP(Pb)3.png|thumb|400px|'''Before Optimization''']]</th>
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</tr></table>
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<table><tr>
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<th>[[Image:T--FAFU-CHINA--Lpp-OmpA-MBP(Pb)4.png|thumb|400px|'''After Optimization''']]</th>
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</tr></table>
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3.GC Content:
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The comparison of  GC content between original sequence and optimized sequence
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<table><tr>
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<th>[[Image:T--FAFU-CHINA--Lpp-OmpA-MBP(Pb)5.png|thumb|400px|'''Before Optimization''']]</th>
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</tr></table>
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<table><tr>
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<th>[[Image:T--FAFU-CHINA--Lpp-OmpA-MBP(Pb)6.png|thumb|400px|'''After Optimization''']]</th>
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</tr></table>
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<table><tr>
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<th>[[Image:T--FAFU-CHINA--Lpp-OmpA-MBP SDS PAGE.png|thumb|400px|'''The expression of Lpp-OmpA-MBP detected using SDS-PAGE ''']]</th>
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</tr></table>
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<table><tr>
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<th>[[Image:T--FAFU-CHINA--M-Lpp-OmpA+MBP(Pb).png|thumb|400px|'''Plasmid digested by PvuII''']]</th>
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</tr></table>
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<table><tr>
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<th>[[Image:T--FAFU-CHINA--GST-CRS5-Toggle-switch.png|thumb|400px|'''Toggle switch ''']]</th>
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</tr></table>
  
 
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Revision as of 17:51, 1 November 2017


Repressor gene xylR+Strong PxylA promoter+RBS+Lpp-Omp-MBP(Pb)+His-tag

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Usage and Biology

Optimization Report

1.Codon Used Adjustment The best value is 1 for sequence optimization.


Before Codon Adjustment
After Codon Adjustment


2.Codon Used Distribution Show the relative codon used distribution

Before Optimization
After Optimization

3.GC Content: The comparison of GC content between original sequence and optimized sequence

Before Optimization
After Optimization
The expression of Lpp-OmpA-MBP detected using SDS-PAGE
Plasmid digested by PvuII
Toggle switch

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 847
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]