Difference between revisions of "Part:BBa K2443008"

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<partinfo>BBa_K2443008 short</partinfo>
 
<partinfo>BBa_K2443008 short</partinfo>
  
Glycine tRNA synthetase responsible for attaching glycine onto its tRNA to form an aminoacyl-tRNA. Codon optimized for use in <i>Escherichia coli</i>. Contains a C-terminal hexahistidine tag with a serine glycine linker. Under the regulation of T7 Promoter, RBS and double terminator.  
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Glycine tRNA synthetase responsible for attaching glycine onto its tRNA to form an aminoacyl-tRNA. Codon optimized for use in <i>Escherichia coli</i>. Contains a C-terminal hexahistidine tag with a serine glycine linker. Under the regulation of T7 Promoter, RBS and double terminator. The expected size of GlyRS beta subunit is 78 kDa, and a band of this size can be seen in lane 10 of the overexpression gel below.
  
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https://static.igem.org/mediawiki/parts/e/e3/BBa_K2443001-Oct_17_gel1.png
  
 
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Revision as of 17:47, 1 November 2017


GlyRS Beta subunit optimized for expression in E. coli

Glycine tRNA synthetase responsible for attaching glycine onto its tRNA to form an aminoacyl-tRNA. Codon optimized for use in Escherichia coli. Contains a C-terminal hexahistidine tag with a serine glycine linker. Under the regulation of T7 Promoter, RBS and double terminator. The expected size of GlyRS beta subunit is 78 kDa, and a band of this size can be seen in lane 10 of the overexpression gel below.

BBa_K2443001-Oct_17_gel1.png

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1214
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1747
    Illegal SapI site found at 104