Difference between revisions of "Part:BBa K2381008"
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<partinfo>BBa_K2381008 short</partinfo> | <partinfo>BBa_K2381008 short</partinfo> | ||
− | It is the first part of split dCas9 which is cut at the amino acid site 713 and 714. | + | It is the first part of split dCas9 which is cut at the amino acid site 713 and 714. This part is the from site 1 to site 713. |
<h3>References</h3> | <h3>References</h3> |
Latest revision as of 17:45, 1 November 2017
Conventional split dCas9-1
It is the first part of split dCas9 which is cut at the amino acid site 713 and 714. This part is the from site 1 to site 713.
References
Nihongaki, Yuta, et al. "Photoactivatable CRISPR-Cas9 for optogenetic genome editing.." Nature Biotechnology 33.7 (2015).
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 1099
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]