Difference between revisions of "Part:BBa K2398000"

Revision as of 17:44, 1 November 2017

Cytochrome c for the synthesis of organosilicons

We present a codon-optimized version of the cytochrome c protein derived from Rhodotermus marinus. This protein is able to perform the conversion of organosilicons. It is a novel catalytic unit that allows utilization of silicon compounds [Arnold et al., 2016]. When in its mature form, it catalyzes a carbene insertion into silicon-hydrogen bonds.

Usage and Biology

The cytochrome c is used for the catalysis of silicon compounds

Characterisation

Figure 1: Gas chromatogram for the reaction of educt (1) and (5) to the product (3). 11.7 minutes retention time, indicates product formation. Unconverted educts converge at 6.9 and 7.2, 7.4 minutes

Figure 2: Mass chromatogram shows the breakdown of the product (3) ethyl 2-((4-aminophenyl)dimethylsilyl)propanoate. The product itself corresponds to a mass of 251 dalton

Figure 3: Gas chromatogram for the reaction of educt (2) and (5) to the product (4). 9.2 minutes retention time indicates product formation.

Figure 4: Mass chromatogram shows the breakdown of the product (4) ethyl 2-(dimethyl(phenyl)silyl)propanoate. The product itself corresponds to a mass of 236 dalton

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BamHI site found at 93
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

@@ Line 10: / Line 10: @@
 ===Characterisation===
-[[File:T--Heidelberg--GCAnilineCompound.png|thumb|center|Figure 1: Gas chromatogram for the reaction of educt (1) and (5) to the product (3). 11.7 minutes retention time, indicates product formation. Unconverted educts converge 6.9 and 7.2, 7.4 minutes]
+[[File:T--Heidelberg--GCAnilineCompound.png|thumb|center|Figure 1: Gas chromatogram for the reaction of educt (1) and (5) to the product (3). 11.7 minutes retention time, indicates product formation. Unconverted educts converge at 6.9 and 7.2, 7.4 minutes]]
 [[File:T--Heidelberg--MSAnilineCompound.png|thumb|center|Figure 2: Mass chromatogram shows the breakdown of the product (3) <i>ethyl 2-((4-aminophenyl)dimethylsilyl)propanoate</i>. The product itself corresponds to a mass of 251 dalton]]