Difference between revisions of "Part:BBa K2381009"
 
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<partinfo>BBa_K2381009 short</partinfo>
 
<partinfo>BBa_K2381009 short</partinfo>
  
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It is the first part of split dCas9 which is cut at the amino acid site 713 and 714.  
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It is the second part of split dCas9 which is cut at the amino acid site 713 and 714. This part is the from site 714 to site 1368.  
  
 
  <h3>References</h3>
 
  <h3>References</h3>

Latest revision as of 17:41, 1 November 2017


Conventional split dCas9-2

It is the second part of split dCas9 which is cut at the amino acid site 713 and 714. This part is the from site 714 to site 1368.

References

Nihongaki, Yuta, et al. "Photoactivatable CRISPR-Cas9 for optogenetic genome editing.." Nature Biotechnology 33.7 (2015).

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1239
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]