Difference between revisions of "Part:BBa K2460001:Design"
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===Design Notes=== | ===Design Notes=== | ||
| − | + | *Note that if you are using a repressor to regulate the integrase, make sure there are already repressors accumulated in the cell to prevent integrase from expressing. | |
| + | *An attB/P site follwing after a promoter may reduce experssion. | ||
| + | *This part has a 6×His tag and a thrombin site, and mutated to be RFC10 compatible. | ||
Latest revision as of 17:39, 1 November 2017
φBT1 integrase
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 180
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 331
Illegal AgeI site found at 1526 - 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI.rc site found at 1489
Design Notes
- Note that if you are using a repressor to regulate the integrase, make sure there are already repressors accumulated in the cell to prevent integrase from expressing.
- An attB/P site follwing after a promoter may reduce experssion.
- This part has a 6×His tag and a thrombin site, and mutated to be RFC10 compatible.
Source
Lab storage