Difference between revisions of "Part:BBa K2381009:Design"
(→Source) |
(→References) |
||
| Line 15: | Line 15: | ||
===References=== | ===References=== | ||
| + | Nihongaki, Yuta, et al. "Photoactivatable CRISPR-Cas9 for optogenetic genome editing.." Nature Biotechnology 33.7 (2015). | ||
Latest revision as of 17:33, 1 November 2017
Conventional split dCas9-2
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 1239
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
The split dCas9 is cut between the amino acid site 713 and 714. This part is the from site 714 to site 1368.
Source
The dCas9 is from Streptococcus pyogenes.
References
Nihongaki, Yuta, et al. "Photoactivatable CRISPR-Cas9 for optogenetic genome editing.." Nature Biotechnology 33.7 (2015).