Difference between revisions of "Part:BBa K2381009:Design"

 
(→‎Design Notes)
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===Design Notes===
 
===Design Notes===
−
the split method is cut it at the loop
 
−
 
  
 +
The split dCas9 is cut between the amino acid site 713 and 714. This part is the from site 714 to site 1368.
  
 
===Source===
 
===Source===

Revision as of 17:30, 1 November 2017


Conventional split dCas9-2


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1239
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The split dCas9 is cut between the amino acid site 713 and 714. This part is the from site 714 to site 1368.

Source

no

References