Difference between revisions of "Part:BBa I763032:Experience"

Revision as of 13:04, 26 October 2007

We cloned the part inside a high copy number plasmid (pSB1AK3. When we checked part lenght with a gel electrophoresis that was ok. Anyway, when we checked for fluorescence we observed that just some bacteria were fluorescent. We grew bacteria in M9 + lactose + IPTG (2 mM),but always we saw that not all of them were fluorescent.

So, we tried tried the same cloning in a low copy number plasmid (pSB4A3), thinking that it could better with a lower amount of cI repressor, but we obtained the same result.

Applications of BBa_I763032

User Reviews

UNIQ33ab705273c82aa3-partinfo-00000000-QINU UNIQ33ab705273c82aa3-partinfo-00000001-QINU

Revision as of 12:58, 26 October 2007 (view source) Francesca ceroni (Talk \| contribs) ← Older edit		Revision as of 13:04, 26 October 2007 (view source) Francesca ceroni (Talk \| contribs) Newer edit →
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	We cloned the part inside a high copy number plasmid (pSB1AK3. When we checked part lenght with a gel electrophoresis that was ok. Anyway, when we checked for fluorescence we observed that just some bacteria were fluorescent. We grew bacteria in M9 + lactose + IPTG (2 mM),but always we saw that not all of them were fluorescent.		We cloned the part inside a high copy number plasmid (pSB1AK3. When we checked part lenght with a gel electrophoresis that was ok. Anyway, when we checked for fluorescence we observed that just some bacteria were fluorescent. We grew bacteria in M9 + lactose + IPTG (2 mM),but always we saw that not all of them were fluorescent.

−	So, we tried tried the same cloning in a low copy number plasmid (pSB4A3), thinking that a it could better with a lower amount of cI repressor, but we obtained the same result.	+	So, we tried tried the same cloning in a low copy number plasmid (pSB4A3), thinking that it could better with a lower amount of cI repressor, but we obtained the same result.