Difference between revisions of "Part:BBa K2381008:Design"
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===Design Notes=== | ===Design Notes=== | ||
| − | the split | + | the split dCas9 is cut between the amino acid site 713 and 714. This part is the from site 1 to site 713. |
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===Source=== | ===Source=== | ||
Revision as of 17:20, 1 November 2017
Conventional split dCas9-1
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 1099
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
the split dCas9 is cut between the amino acid site 713 and 714. This part is the from site 1 to site 713.
Source
The dCas9 is from Streptococcus pyogenes.
References
Nihongaki, Yuta, et al. "Photoactivatable CRISPR-Cas9 for optogenetic genome editing.." Nature Biotechnology 33.7 (2015).