Difference between revisions of "Part:BBa I763019:Experience"

 
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This experience page is provided so that any user may enter their experience using this part.<BR>Please enter
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how you used this part and how it worked out.
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We cloned the part inside a high copy number plasmid (pSB1AK3. When we checked part lenght with a gel electrophoresis that was ok. Anyway, when we checked for fluorescence we observed that just some bacteria were fluorescent. We grew bacteria in M9 + lactose + IPTG (2 mM),but always we saw that not all of them were fluorescent.
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So, we tried tried the same cloning in a low copy number plasmid (pSB4A3), thinking that a it could better with a lower amount of cI repressor, but we obtained the same result.
  
 
===Applications of BBa_I763019===
 
===Applications of BBa_I763019===

Revision as of 13:01, 26 October 2007


We cloned the part inside a high copy number plasmid (pSB1AK3. When we checked part lenght with a gel electrophoresis that was ok. Anyway, when we checked for fluorescence we observed that just some bacteria were fluorescent. We grew bacteria in M9 + lactose + IPTG (2 mM),but always we saw that not all of them were fluorescent.

So, we tried tried the same cloning in a low copy number plasmid (pSB4A3), thinking that a it could better with a lower amount of cI repressor, but we obtained the same result.

Applications of BBa_I763019

User Reviews

UNIQf4d0ba1b8e829808-partinfo-00000000-QINU UNIQf4d0ba1b8e829808-partinfo-00000001-QINU