Difference between revisions of "Part:BBa K2381008:Design"

 
(References)
Line 16: Line 16:
  
 
===References===
 
===References===
 +
Nihongaki, Yuta, et al. "Photoactivatable CRISPR-Cas9 for optogenetic genome editing.." Nature Biotechnology 33.7 (2015).

Revision as of 17:09, 1 November 2017


Conventional split dCas9-1


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1099
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

the split method is cut it at the loop


Source

no

References

Nihongaki, Yuta, et al. "Photoactivatable CRISPR-Cas9 for optogenetic genome editing.." Nature Biotechnology 33.7 (2015).