Difference between revisions of "Part:BBa K2381008"

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It is the first part of split dCas9 which is cut at the amino acid site 713 and 714.  
 
It is the first part of split dCas9 which is cut at the amino acid site 713 and 714.  
  
   
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  <h3>References</h3>
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Nihongaki, Yuta, et al. "Photoactivatable CRISPR-Cas9 for optogenetic genome editing.." Nature Biotechnology 33.7 (2015).
  
 
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Revision as of 17:07, 1 November 2017


Conventional split dCas9-1

It is the first part of split dCas9 which is cut at the amino acid site 713 and 714.

References

Nihongaki, Yuta, et al. "Photoactivatable CRISPR-Cas9 for optogenetic genome editing.." Nature Biotechnology 33.7 (2015).

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1099
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]