Difference between revisions of "Part:BBa K2317006:Design"
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===Design Notes=== | ===Design Notes=== | ||
| − | + | 2 of the 5 mutation sites are nonsense mutations, just to avoid restriction site. | |
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===Source=== | ===Source=== | ||
Revision as of 17:06, 1 November 2017
DmpR
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 86
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 564
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 249
Illegal BsaI.rc site found at 790
Illegal SapI.rc site found at 1447
Design Notes
2 of the 5 mutation sites are nonsense mutations, just to avoid restriction site.
Source
genebank
References
1.Arlene A. Wise and Cheryl R. Kuske. Generation of novel bacterial regulatory proteins that detect priority pollutant phenols. Appl. Environ. Microb. 2000, 163-169