Difference between revisions of "Part:BBa K2381007"
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<partinfo>BBa_K2381007 short</partinfo> | <partinfo>BBa_K2381007 short</partinfo> | ||
| − | It is the other part of rational designed split dCas9. The design is from split-Cas9 enzyme in which Cas9 is split into two lobes according to its function. This part of dCas9 is a α-helical lobe corresponding to Cas9. The dCas9 is from Streptococcus pyogenes. | + | It is the other part of rational designed split dCas9. The design is from split-Cas9 enzyme in which Cas9 is split into two lobes according to its function. This part of dCas9 is a α-helical lobe corresponding to Cas9 and it is cut at amino acid site 56 and 714. The dCas9 is from Streptococcus pyogenes. |
<h3>References</h3> | <h3>References</h3> | ||
Revision as of 16:51, 1 November 2017
Rational split dCas9-2
It is the other part of rational designed split dCas9. The design is from split-Cas9 enzyme in which Cas9 is split into two lobes according to its function. This part of dCas9 is a α-helical lobe corresponding to Cas9 and it is cut at amino acid site 56 and 714. The dCas9 is from Streptococcus pyogenes.
References
Wright, Addison V., et al. "Rational design of a split-Cas9 enzyme complex." Proceedings of the National Academy of Sciences of the United States of America 112.10 (2015): 2984-2989.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 934
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]