Difference between revisions of "Part:BBa K2384002"

Revision as of 16:47, 1 November 2017

Lpp-OmpA-MBP(Hg)

It is highly expected that when fused with N-terminal fragment of Outer membrane protein A (OmpA), the mercury metal binding peptide(MBP)will be translocated and displayed on the surface of bacteria, which will contribute a lot to increase the mental binding capacity when combined will other collection parts. In the Lpp-Omp display system with potential for displaying macromolecular proteins,it contains signal peptide and the first nine amino acids of Lpp and forty-sixth to 159th amino acids of OmpA.The former contains all the information that carries the protein to the outer membrane and anchors it to the inside of the outer membrane,and the latter can anchor the target fusion protein to the outside of the membrane.The MBP that follows it can be expose. Our sequence optimization is based on the sequence provided by Peking University's IGEM VIKI in 2010.It is more suitable for expression in Bacillus megaterium.

Optimization Report

1.Codon Used Adjustment The best value is 1 for sequence optimization.

Before Codon Adjustment

After Codon Adjustment

2.Codon Used Distribution Show the relative codon used distribution

Before Optimization

After Optimization

3.GC Content: The comparison of GC content between original sequence and optimized sequence

Before Optimization

After Optimization

The expression of Lpp-OmpA-MBP detected using SDS-PAGE

Plasmid digested by EcoRI-PstI

Sequence and Features