Difference between revisions of "Part:BBa K2381007:Design"
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===Design Notes=== | ===Design Notes=== | ||
| − | the split method is | + | the split method is cutting dCas9 at amino acid site 56 and 714. |
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===Source=== | ===Source=== | ||
Latest revision as of 16:39, 1 November 2017
Rational split dCas9-2
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 934
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
the split method is cutting dCas9 at amino acid site 56 and 714.
Source
The dCas9 is from Streptococcus pyogenes.
References
Wright, Addison V., et al. "Rational design of a split-Cas9 enzyme complex." Proceedings of the National Academy of Sciences of the United States of America 112.10 (2015): 2984-2989.