Difference between revisions of "Part:BBa K2381007"
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It is the other part of rational designed split dCas9. The design is from split-Cas9 enzyme in which Cas9 is split into two lobes according to its function. This part of dCas9 is a α-helical lobe corresponding to Cas9. | It is the other part of rational designed split dCas9. The design is from split-Cas9 enzyme in which Cas9 is split into two lobes according to its function. This part of dCas9 is a α-helical lobe corresponding to Cas9. | ||
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| + | <h3>References</h3> | ||
| + | Wright, Addison V., et al. "Rational design of a split-Cas9 enzyme complex." Proceedings of the National Academy of Sciences of the United States of America 112.10 (2015): 2984-2989. | ||
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Revision as of 16:27, 1 November 2017
Rational split dCas9-2
It is the other part of rational designed split dCas9. The design is from split-Cas9 enzyme in which Cas9 is split into two lobes according to its function. This part of dCas9 is a α-helical lobe corresponding to Cas9.
References
Wright, Addison V., et al. "Rational design of a split-Cas9 enzyme complex." Proceedings of the National Academy of Sciences of the United States of America 112.10 (2015): 2984-2989.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 934
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]