Difference between revisions of "Part:BBa K2474000:Design"

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===Design Notes===
 
===Design Notes===
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We designed this plasmids with amyloid-beta bound to the fluorescent protein mNeonGreen with C-terminus. For this plasmid we used an arabinose promotor called AraC which is induced by addition of arabinos. To connect the fusion proteins we had to create a GS-linker between Amyloid-Beta and mNeonGreen to make it more motile. The fusion protein carried a histidine tag to enable IMAC analysis.
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We designed this biobrick with amyloid-beta bound C-terminally to the fluorescent protein mNeonGreen. For this plasmid we used the arabinose induced promotor BBa_I0500 which includes the regulatory protein araC which binds to the promoter-region pBAD in the absence of arabinose. To connect the fusion proteins we created a GS-linker between Amyloid-Beta and mNeonGreen to make sure it was motile. The fusion protein carries a histidine tag (N-terminally) to enable IMAC purification.
  
 
===Source===
 
===Source===

Revision as of 16:22, 1 November 2017


Amyloid-B mNeonGreen


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1205
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1267
    Illegal BamHI site found at 1144
    Illegal BamHI site found at 2190
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 979
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 961


Design Notes

We designed this biobrick with amyloid-beta bound C-terminally to the fluorescent protein mNeonGreen. For this plasmid we used the arabinose induced promotor BBa_I0500 which includes the regulatory protein araC which binds to the promoter-region pBAD in the absence of arabinose. To connect the fusion proteins we created a GS-linker between Amyloid-Beta and mNeonGreen to make sure it was motile. The fusion protein carries a histidine tag (N-terminally) to enable IMAC purification.

Source

mNeonGreen and Amyloid-Beta was synthetically made by the company IDT. pSB1C3 came form iGEM and pBAD is a BioBrick (BBa_10500).

References