Difference between revisions of "Part:BBa K2302007"
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<partinfo>BBa_K2302007 short</partinfo> | <partinfo>BBa_K2302007 short</partinfo> | ||
| − | This plasmid cords for lipase out the membrane,which can hydrolyze fat to fatty acids, it consists three basic parts: | + | This plasmid cords for lipase out the membrane,which can hydrolyze fat to fatty acids, it consists three basic parts: His, PelB and CALB. PelB is sequence cords for a functional signal peptide, which transport the fused protein CALB out the membrane efficiently, and CALB is a kind of lipase which can hydrolyze fat. By transforming this plasmid, a bacteria can secret lipase out the membrane,hydrolyzing fat in the environment to fatty acids. |
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Latest revision as of 16:21, 1 November 2017
His+PelB+CALB
This plasmid cords for lipase out the membrane,which can hydrolyze fat to fatty acids, it consists three basic parts: His, PelB and CALB. PelB is sequence cords for a functional signal peptide, which transport the fused protein CALB out the membrane efficiently, and CALB is a kind of lipase which can hydrolyze fat. By transforming this plasmid, a bacteria can secret lipase out the membrane,hydrolyzing fat in the environment to fatty acids.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 1050
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 52
Illegal NgoMIV site found at 78
Illegal NgoMIV site found at 771
Illegal NgoMIV site found at 881
Illegal AgeI site found at 573
Illegal AgeI site found at 831 - 1000COMPATIBLE WITH RFC[1000]