Difference between revisions of "Part:BBa K2381006"
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<partinfo>BBa_K2381006 short</partinfo> | <partinfo>BBa_K2381006 short</partinfo> | ||
| − | It is a part of rational designed split dCas9. The design is from split-Cas9 enzyme in which Cas9 is split into two lobes according to its function. This part of dCas9 is a nuclease lobe corresponding to Cas9. | + | It is a part of rational designed split dCas9. The design is from split-Cas9 enzyme in which Cas9 is split into two lobes according to its function. This part of dCas9 is a nuclease lobe corresponding to Cas9. The dCas9 is cut at the end of amino acid 57 and the start of amino acid 729, then connected with a GSS linker. |
<h3>References</h3> | <h3>References</h3> | ||
Latest revision as of 16:21, 1 November 2017
Rational split dCas9-1
It is a part of rational designed split dCas9. The design is from split-Cas9 enzyme in which Cas9 is split into two lobes according to its function. This part of dCas9 is a nuclease lobe corresponding to Cas9. The dCas9 is cut at the end of amino acid 57 and the start of amino acid 729, then connected with a GSS linker.
References
Wright, Addison V., et al. "Rational design of a split-Cas9 enzyme complex." Proceedings of the National Academy of Sciences of the United States of America 112.10 (2015): 2984-2989.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 1374
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]