Difference between revisions of "Part:BBa K2378006"

 
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<partinfo>BBa_K2378006 short</partinfo>
 
<partinfo>BBa_K2378006 short</partinfo>
  
This is optimized coding sequence of PETase enzyme for E. coli BL21 with the addition of NhaR BBa_K1357002 (https://parts.igem.org/wiki/index.php?title=Part:BBa_K1357002) coding sequence. This part also use constitutive promoters BBa_J23106.
+
This is optimized coding sequence of PETase enzyme for <i>E. coli</i> BL21 with the addition of NhaR BBa_K1357002 (https://parts.igem.org/wiki/index.php?title=Part:BBa_K1357002) coding sequence. This part also use constitutive promoters BBa_J23106.
  
  

Revision as of 16:18, 1 November 2017


NhaR-PETase Constitutive Coding Device

This is optimized coding sequence of PETase enzyme for E. coli BL21 with the addition of NhaR BBa_K1357002 (https://parts.igem.org/wiki/index.php?title=Part:BBa_K1357002) coding sequence. This part also use constitutive promoters BBa_J23106.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
    Illegal NotI site found at 1002
    Illegal NotI site found at 1911
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1656
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 236