Difference between revisions of "Part:BBa K2378006"
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<partinfo>BBa_K2378006 short</partinfo> | <partinfo>BBa_K2378006 short</partinfo> | ||
− | This is optimized coding sequence of PETase enzyme for E. coli BL21 with the addition of NhaR BBa_K1357002 (https://parts.igem.org/wiki/index.php?title=Part:BBa_K1357002) coding sequence. This part also use constitutive promoters BBa_J23106. | + | This is optimized coding sequence of PETase enzyme for <i>E. coli</i> BL21 with the addition of NhaR BBa_K1357002 (https://parts.igem.org/wiki/index.php?title=Part:BBa_K1357002) coding sequence. This part also use constitutive promoters BBa_J23106. |
Revision as of 16:18, 1 November 2017
NhaR-PETase Constitutive Coding Device
This is optimized coding sequence of PETase enzyme for E. coli BL21 with the addition of NhaR BBa_K1357002 (https://parts.igem.org/wiki/index.php?title=Part:BBa_K1357002) coding sequence. This part also use constitutive promoters BBa_J23106.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30
Illegal NotI site found at 1002
Illegal NotI site found at 1911 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 1656
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 236