Difference between revisions of "Part:BBa K2302010"

Latest revision as of 16:09, 1 November 2017

T7-His-PelB+CALB+T7terminater

This plasmid expresses a fusion protein containing a lipase part and can be transported out the of bacterial membrane. The fusion protein can hydrolyze fat to fatty acids. The expression plasmid consists of four basic parts: the T7 promoter, His, PelB and CALB. PelB is a sequence encoding a functional signal peptide that helps the CALB fusion protein to be transported out of the membrane. CALB is a lipase that can hydrolyze fat. Being transformed with this plasmid, the host bacteria can secrete lipase out the membrane to hydrolyze fat in the environment to fatty acids.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BamHI site found at 1079
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal NgoMIV site found at 81
Illegal NgoMIV site found at 107
Illegal NgoMIV site found at 800
Illegal NgoMIV site found at 910
Illegal AgeI site found at 602
Illegal AgeI site found at 860
1000
COMPATIBLE WITH RFC[1000]

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 <partinfo>BBa_K2302010 short</partinfo>
-this plasmid expresses a fusion protein containing a lipase part and can be transported out the of bacterial membrane. The fusion protein can hydrolyze fat to fatty acids. The expression plasmid consists of four basic parts: the T7 promoter, His, PelB and CALB. PelB is a sequence encoding a functional signal peptide that helps the CALB fusion protein to be transported out of the membrane. CALB is a lipase that can hydrolyze fat. Being transformed with this plasmid, the host bacteria can secrete lipase out the membrane to hydrolyze fat in the environment to fatty acids.
+This plasmid expresses a fusion protein containing a lipase part and can be transported out the of bacterial membrane. The fusion protein can hydrolyze fat to fatty acids. The expression plasmid consists of four basic parts: the T7 promoter, His, PelB and CALB. PelB is a sequence encoding a functional signal peptide that helps the CALB fusion protein to be transported out of the membrane. CALB is a lipase that can hydrolyze fat. Being transformed with this plasmid, the host bacteria can secrete lipase out the membrane to hydrolyze fat in the environment to fatty acids.
 <!-- Add more about the biology of this part here