Difference between revisions of "Part:BBa K2243010"
Line 6: Line 6:
  
 
We use this part to be the state switch of the flip-flop. See the link of the bio-flip-flop.
 
We use this part to be the state switch of the flip-flop. See the link of the bio-flip-flop.
 +
 +
<html>
 +
<body>
 +
 +
<img src="https://static.igem.org/mediawiki/2017/f/fc/Peking_flipflop_fig_5.svg"  height="500" width="600"/>
 +
 +
</body>
 +
</html>
 +
 +
Figure 2. The standard testing system used to characterize the recombinases.
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Revision as of 16:06, 1 November 2017


TP901-1 attB_J23119_TP901-1 attP

The constitutive promoter J23119 is between Bxb1 gp35 specific sites and will be inverted by the Bxb1 gp35 recombinase, thus leading to the direction of promoter and change of gene expression. This unit can be used as standard reporter of recombinase bxb1 gp35 characterization.

We use this part to be the state switch of the flip-flop. See the link of the bio-flip-flop.

Figure 2. The standard testing system used to characterize the recombinases.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 105
    Illegal NheI site found at 128
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 188
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]